![]() 8, 9 The complementarity-determining regions (CDRs), CDR1, CDR2 and CDR3, as well as the surrounding framework regions, together form the antigen-binding site of the antibody. This Review provides an overview of technologies for large-scale sequencing of antibody repertoires, and discusses how these technologies can be applied to characterize immune responses and identify antibodies of therapeutic, diagnostic or mechanistic relevance to autoimmune diseases, including rheumatic diseases.Īntibodies are comprised of an immunoglobulin heavy chain (IgH) and light chain (IgL), each containing an antigen-binding domain that is generated by the recombination, junctional diversification and somatic hypermutation of variable (V), joining (J) and/or diversity (D) gene segments during B-cell development. 4, 6, 7 A challenge in understanding, as well as in diagnostically and therapeutically harnessing, antibody responses is the identification of antibodies that underlie functional immune responses, that is, antibodies that directly contribute to an immune outcome, such as neutralizing a microbial pathogen or mediating autoimmune tissue injury. 1, 2, 3 In infectious diseases, in which antibody responses are usually protective, there is growing interest in isolating antibodies that could be developed as novel therapeutic agents 4, 5 and in using microbial antigens and epitopes targeted by antibody responses to develop vaccines. In autoimmune diseases, including autoimmune rheumatic diseases, antibody characterization has enabled the identification of autoantigens and has provided insights into the underlying mechanisms of disease furthermore, detection of autoantibodies has become a cornerstone of modern diagnostics. ![]() Analysis of these antibody repertoires, particularly those contributing to functional immune responses, can provide important information on protective and pathogenic immunity. In response to microbial infection, vaccination, autoimmune disease or cancer, the immune system generates distinct antibody repertoires. We anticipate that antibody repertoire sequencing will provide next-generation biomarkers, diagnostic tools and therapeutic antibodies for a spectrum of diseases, including rheumatic diseases.Īntibodies are a major component of the adaptive immune system and have critical roles in protective and pathogenic immune responses. Sequencing antibody repertoires is transforming our understanding of immune responses to autoimmunity, vaccination, infection and cancer. Integrated analysis of coexpressed functional genes provides the potential to further pinpoint the most important antibodies and clonal families generated during an immune response. Bioinformatic identification of clonal antibody families and recombinant expression of representative members produces recombinant antibodies that can be used to identify the antigen targets of functional immune responses and to investigate the mechanisms of their protective or pathogenic functions. Although focused sequencing of immunoglobulin heavy chains or complement determining regions can be utilized to monitor particular immune responses and B-cell malignancies, high-fidelity analysis of the full-length paired heavy and light chains expressed by individual B cells is critical for characterizing functional antibody repertoires. Important parameters to consider when sequencing antibody repertoires include the methodology, the B-cell population and clinical characteristics of the individuals analysed, and the bioinformatic analysis. ![]() ![]() The development of high-throughput DNA sequencing technologies has enabled large-scale characterization of functional antibody repertoires, a new method of understanding protective and pathogenic immune responses. ![]()
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![]() ![]() Dudley: (excited) "Oh, you're all mine.(Dudley opens the doughnut box and fixes his eyes onto the gleaming chocolate doughnut inside).(elevator doors shut and indicator light flashes Dudley quickly runs up the stairs, leaps through a dog door and spins in the air until he lands in front of a doughnut box sitting on a table).NON RESERVED VISITOR PARKING!" ( presses elevator button) to level 6": (shows "6: Reserved Visitor Parking" button with "NON" written in red next to Reserved ). Dudley: (just outside of elevator bold) "Wrong, Kitty! 'Cause you're goin' down.(smiles evilly) And that doughnut is mine! Kitty: (unhelmeted smirks) "Sorry Dudley, we both know there's only one chocolate doughnut in the snack room.(the motorcyclist slides into the elevator and comes to a halt she removes her helmet and unfurls her hair to reveal herself as Kitty).(as the garage's door begins to close, the rider oversteers and powerslides to sneak underneath the closing door they continue to slide past Dudley, who is desperately trying to run towards something in the distance).Mobile just inside the building and he jumps out to make a run for it) headquarters Dudley speedily lands the T.U.F.F. Mobile's airplane mode the engines thrust and the vehicle rockets through the air, well past the motorcyclist and into the indoor car-park of the T.U.F.F. ![]() (.and fall off the end of an elevated highway while falling through the air, Dudley presses another big red dashboard button to activate the T.U.F.F.Dudley: (losing control) " Whooooooooooaaaaaaaaaaa!".(the rider lands in front of Dudley and continues driving the motorcycle releases an oil slick which causes the T.U.F.F. ![]() Mobile they fly into the motorcycle and explode, only to release enough energy to cause the rider to launch into the air and fly over Dudley)
![]() ![]() It runs on both 32-bit and 64-bit systems with no dedicated 64-bit download provided. Previous versions of the operating system shouldn't be a problem with Windows 8, Windows 7 and Windows Vista having been tested. ![]() What version of Windows can DVD Cloner run on?ĭVD Cloner can be used on a computer running Windows 11 or Windows 10. The DVD Cloner 2013 10.70 demo is available to all software users as a free download with potential restrictions and is not necessarily the full version of this software. It also works as DVD/Blu-ray player software for you to preview every movie title to avoid unexpected errors and mistakes. This download is licensed as shareware for the Windows operating system from disc utilities and can be used as a free trial until the trial period ends (after an unspecified number of days). For professional users, DVD-Cloner 2023 also enables you to make movie complete copy, movie-only copy, split copy or customized copy to satisfy everyone all over the world.
![]() ![]() You can learn more about the organization from this article at Huffington Post. ![]() The underlying basis of the group is to shun contraception as they believe God will only give them as many children as they can handle. Some Quiverfull members reportedly believe they are meant to raise a conservative Christian army. One documentary subject describes them as looking like the “perfect Aryan clan.” And if you look at all the siblings Cline produced, they are almost all blue-eyed blonds. It wasn’t just the “breeding” angle that drew their attention but the fact members of Quiverfull were allegedly careful to only produce white children due to fears they would one day disappear. The verse, “Before I formed you in your mother’s womb, I knew you,” is one that Cline frequently quoted. One of the reasons Jacoba Ballard and her half-siblings thought Cline was connected to the group is because they saw the Bible verse Jeremiah 1:5 used on the website. In Our Father, Quiverfull is described as an organization that would feel right at home in The Handmaids Tale, where women are seen as breeders to create more followers of the verse and indoctrinate others. It’s a cult-like group where the goal is to have as many children as possible and then send those kids out into the world like “arrows” (a “quiver” is a term that refers to a case archers use to hold their arrows) to spread the word of the Bible. Our Father: What is the Quiverfull movement? Below we’ll discuss what the movement actually is and whether or not Cline was actually part of it. ![]() The movement is mentioned once again in this documentary, this time with an alleged connection to Cline. One disturbing reveal from the documentary is a theory that Cline was involved with a religious movement (or cult) called Quiverfull.Ĭonsidered a hardcore religious sect devoted to procreating as much as possible to create more Christians devoted to biblical civil law, Quiverfull received a lot of attention due to the many scandals surrounding the Duggar family. The truth about former fertility doctor Donald Cline is horrifying enough, but the new Netflix documentary Our Father further explores Cline’s misdeeds and the impact it has had on his children. ![]() ![]() The single reached number 49 on the pop charts. ![]() In 1957, Simon & Garfunkel recorded the song "Hey, Schoolgirl" under the name Tom & Jerry. Their idols were The Everly Brothers, whom they imitated in their use of close two-part vocal harmony. ![]() īetween 19, the two performed together as "Tom & Jerry", a moniker coined by their label Big Records, occasionally performing at school dances. It has been said by Garfunkel that Simon first became interested in singing after hearing Garfunkel sing a rendition of Nat King Cole's " Too Young" in a school talent show. Just 3 against 3, half court pick up games in the schoolyard." He met future singing partner Paul Simon in the sixth grade at PS 164, when they were both cast in the elementary school graduation play, Alice in Wonderland. Then 102! I never played on a team after junior high school. Much of the summer I spent methodically hitting 96, 98 foul shots out of 100. I couldn't run around, but I loved basketball and there was a hoop nearby. He explained in a 1998 interview: "In the summer of '55, I had a lung infection. As a young teen, Garfunkel became ill with a lung infection, leading to a love for basketball. ![]() "When we were lined up in size order, and after everyone else had left, I'd stay behind and enjoy the echo sound of the stairwell tiles and sing ' Unchained Melody' and ' You'll Never Walk Alone', learning to love this goosebumps song from the tender age of five." Later, Garfunkel's father bought him a wire recorder and from then on, Garfunkel spent his afternoons singing, recording, and playing it back, so he could listen for flaws and learn how to improve.Īt his bar mitzvah in 1954, Garfunkel performed as a cantor, singing over four hours of his repertoire for his family. His maternal cousin was Lou Pearlman, founder of the Backstreet Boys and NSYNC.Īccording to the Across America DVD, Garfunkel's love of singing originated in the first grade. When he was young, he would often sing in synagogue. Garfunkel is of Romanian-Jewish descent, his paternal grandparents having emigrated from the city of Iași. Before his career in sales, Jacob worked as an actor in Dayton, Ohio. Jacob's parents immigrated to the United States at the beginning of the twentieth century, and settled in Manhattan. Art was a middle child with two brothers, the older Jules and the younger Jerome. Garfunkel was born in Forest Hills, Queens, New York City, to Rose (born Pearlman) and Jacob "Jack" Garfunkel, a traveling salesman. In 2008, Garfunkel was ranked 86th in Rolling Stone magazine's list of the 100 Greatest Singers of All Time. In 1990, he and Simon were inducted into the Rock and Roll Hall of Fame. Through his solo and collaborative work, Garfunkel has earned eight Grammy Awards, including a Lifetime Achievement Award. Highlights of Garfunkel's solo music career include one top 10 hit, three top 20 hits, six top 40 hits, 14 Adult Contemporary top 30 singles, five Adult Contemporary number ones, two UK number ones and a People's Choice Award. Both men experienced success in solo careers in the years following the duo's breakup. Simon & Garfunkel split for personal reasons, but the pair have occasionally reunited in the years since. ![]() Robinson" (written for the 1967 film The Graduate), " Scarborough Fair", The Boxer and " Bridge over Troubled Water", whose title also served as the name of Simon & Garfunkel's final album in 1970. Their combined presence in music began in the 1950s, and throughout the 1960s, the duo of Simon & Garfunkel achieved great chart success with tracks such as " The Sound of Silence", " Mrs. Born in Forest Hills, Queens, New York, Garfunkel became acquainted with Simon through an elementary school play of Alice in Wonderland and sought a partnership. Arthur Ira Garfunkel (born November 5, 1941) is an American singer and actor who is best known for his partnership with Paul Simon in the folk rock duo Simon & Garfunkel. ![]() ![]() The new Mercedes-Benz Group is thus fully concentrating on its passenger car brands Mercedes-Benz, Mercedes-AMG, Mercedes-Maybach and Mercedes-EQ as well as vans. On December 10, 2021, Daimler Truck Holding AG was launched as an independent company on the Frankfurt Stock Exchange. With the renaming of Daimler, the company completes its historic realignment that began last year and has been approved by the shareholders with an overwhelming majority. Under the name Mercedes-Benz Mobility AG, doing business as Mercedes-Benz Financial Services in the U.S., the company offers mobility services for passenger cars and vans in the areas of financing, leasing and insurance. Parallel to the renaming of Daimler AG, Daimler Mobility AG is also adapting its brand. ![]() We want to continue this legacy of our founders by taking the lead in electric mobility and vehicle software." The Mercedes star has always been a promise for the future: Changing the present in order to improve it. In doing so, we want to make clear where we see the core of our company – building the most desirable cars in the world. Ola Källenius, Chairman of the Board of Management of Mercedes-Benz Group AG: "The renaming to Mercedes-Benz Group AG underlines our renewed strategic focus. The shares of the Mercedes-Benz Group AG will remain listed on Germany’s blue-chip DAX stock index. There will be no further changes for the shareholders. ![]() In the course of the renaming of Daimler AG to Mercedes-Benz Group AG, the company's stock exchange symbol also changes from ✽AI« to »MBG«. The two pioneers had already presented their ground-breaking inventions independently of each other in 1886, heralding the beginning of the automotive age. The world-famous brand Mercedes-Benz was created in 1926 when the predecessor companies of Carl Benz and Gottlieb Daimler were merged with the aim of revolutionizing automobile production. Following the successful stock market debut of Daimler Truck, the Group’s renewed focus on the automotive business is being underlined with a new name. What do you think of the rebranding? Do you think it will be helpful for Mercedes-Benz? Let us know in the comments.STUTTGART, Germany-( BUSINESS WIRE)-Daimler AG will become Mercedes-Benz Group AG on February 1, 2022. That may be a limitation on how far the multiple could go near-term.” “For Mercedes-Benz, you have to convert your existing ICE business to EVs. “Lucid and Tesla get to start at 100 percent EV,” said Tom Narayan, European auto analyst at RBC Capital Markets. Other senior Daimler figures believe the move has come at the right time to “sustainably safeguard success”.īernd Pischetsrieder, Daimler’s supervisory board chairman, said, “By spinning off the commercial-vehicle business, we aim to create value for our shareholders, increase our profitability and fully exploit our potential.”Īutomotive News Europe reported that some analysts believe Mercedes-Benz will be viewed similarly to Tesla or Lucid, targeting an EV multiple (an indicator that can be used to value stock), but suggested that a major challenge for the firm will be the shift from internal combustion engines. Its all-electric EQ range already contains six models: the EQC SUV, the EQA and EQB crossovers, the EQS limousine, the EQV MPV and the EQE sedan. “If we can boost cash flow and our multiples, there’s lots of potential in the Mercedes-Benz stock,” he said. Daimler's shift to electric vehicles is already well under way. Going forward, it will focus on autonomous driving, intelligent connectivity and new mobility concepts. ![]() Daimler is currently worth around €77 billion (over Rs 6,500 crore), but Kallenius didn't suggest a specific valuation target for the firm. Kallenius hopes the move will potentially unlock shareholder value and “unlock the full potential” of both companies as leaders in automotive innovation. ![]() ![]() ![]()
![]() ![]() Unfortunately an implementation of AirPlay 2 isn’t yet present in this early version of Control Center, and it still has its fair share of quirks. ![]() ![]() Traditionalists will be happy to know that you can drag elements from the Control Center block of controls directly into the menu bar for quick shortcuts access. Mac users will be able to control volume, screen brightness, keyboard backlight, Bluetooth and Wifi, and other functions directly from Control Center in Big Sur. ![]() Control Center, like its iOS counterpart, allows users to control many system functions within a common area. The implementation of Control Center into macOS is finally here in Big Sur, and its closely tied to the traditional macOS menu bar. In Messages, the update is even more useful, with the ability to use Memoji stickers, insert GIF animations, use Message effects, inline replies, pinned messages, rename groups, and more. The enhancements mean that you’ll now be able to use the Street View-like Look Around feature in Maps, use Guides, and access indoor maps, among other improvements. Now both Maps and Messages more or less gain parity with their iOS counterparts. These apps, which are based on their iPad versions, are massive improvements from a functionality standpoint over the Maps and Messages apps found in macOS Catalina. In macOS Big Sur we gain two more native Mac Catalyst apps in Messages and Maps. Watch our video walkthrough embedded above for a hands-on look. Thanks to macOS Big Sur, I now have one less reason to use Google’s browser.įinally, there’s a new Custom Start Page, which allows you to add a background image, Privacy Report, iCloud Tabs, Frequently Visited sites, Reading List, and more. In years past, the lack of a built-in Safari translation tool caused me to use Chrome from time to time. There’s now a native translation utility baked into Safari, just like you’ll find in iOS 14. Website previews allow you to hover your cursor over a tab to view a quick preview of what the tab contains. Favicons on tabs are now enabled by default, and when you have lots of open tabs, Favicons become the tool to help you quickly identify them.Īll new in the latest version of Safari are website previews. Safari in Big Sur is also focused on the user experience when it comes to managing lots of tabs. These changes shouldn’t evoke surprise, as Safari has always been the most efficient browser for Mac users. Apple notes that frequently-visited pages load up to 50% faster than competing browsers, and users can stream video for up to three hours longer and browse the web for up to one hour longer. Safari is even faster and more power efficient in macOS Big Sur. Oh, and the wonderful startup sound that Apple disabled a few years back has made a return it can now be toggled via Sound preferences. The new sounds complement the new UI in that they seem a bit more lighthearted and airy. The updated sound effects are a breath of fresh air for those of us who’ve grown tired of the typical macOS sounds. Although many of the app Icons feature unique textures and Mac-like stylistic elements, the updated rounded rectangle icons are unmistakably influenced by iOS. Outside of the new floating translucent dock and translucent menu bar, the most noticeable visual change involves app icons. Subscribe to 9to5mac on YouTube for more videos Thanks to MacPaw for sponsoring 9to5mac on YouTube: Get CleanMyMac X with a 30% discount Video: macOS Big Sur changes and features Although full uniformity isn’t all the way there yet in this initial beta, I suspect that future betas will continue to bring apps and utilities in line with macOS’ new design language. The new side bar design lends more space for frequently used elements within your favorite apps.Īnother big change are the UI symbols, which give apps a more consistent look. One of the biggest changes that you’ll notice are the full-height sidebars, which are found in the Finder and within apps like Notes, Reminders, Music, etc. Watch our hands-on video walkthrough as we discuss over 85 macOS Big Sur changes and features, and be sure to subscribe to 9to5mac on YouTube for more videos. Everything, from the dock, to the menu bar, to window chrome, icons, and even sounds have been updated. The biggest takeaway from my hands-on time with the follow up to macOS Catalina is that Apple’s latest OS is clearly being designed with the future in mind.Īlthough it’s unmistakably Mac, Big Sur is a departure from previous versions of macOS in terms of aesthetics. After going in depth with iOS 14 earlier this week, today we focus on macOS Big Sur. ![]() ![]() Any website or app that enjoys a good share of European audience knows that having different languages makes the content incredibly more engaging for a big part of those users. With accessibility becoming a standard for responsive design, we have built a market-proven solution that not only offers UK English text to speech, but also an abundance of other languages. The WCAG 2.0 standard was established to solve that problem, and we are happy to make our part. Speechnotes is a reliable and secure web-based speech-to-text tool that enables you to quickly and accurately transcribe your audio and video recordings, as well as dictate your notes instead of typing, saving you time and effort. The current browsing experience puts many obstacles in front of them, becoming needlessly difficult and frustrating. ResponsiveVoice can help make your website WCAG 2.0 compliant, especially for people who are print disabled, and those who suffer from a variety of impediments including dyslexia, vision impairment, and quadriplegia. Most legislature refers to the Web Content Accessibility Guidelines 2.0 – and in particular their AA level – as the sensible set of guidelines to follow, until official legislation covers all content published on the web. ![]() Each state has its own set of guidelines (in the United Kingdom, for instance, they were established with the Equality Act of 2010, and Web Accessibility standards were defined through BS 8878:2010, the Web Accessibility Code of Practice), but for online content next to no state has worked out the details. AccessibilityĪccessibility guidelines are here to stay, to create websites and apps that are better for everyone. The two female voices sound very similar, so that users can have a consistent experience on platforms that are officially supported, but also on those that are not. The fallback voice is female, and is used in the Android default browser, Internet Explorer, Firefox, and Opera. is a FREE online Text-to-Speech (TTS Free) website based on AI technology. The UK English Female voice is also used in browsers that do not natively support both versions, such as Internet Explorer, Firefox, and Opera. ResponsiveVoice UK English text to speech (both male and female) is supported natively in Chrome desktop, Safari, and iOS. Chrome on Android does support UK English Male in the operating system, but it is not accessible to the browser, so ResponsiveVoice falls back to UK English Female as the best case available. ResponsiveVoice gets you an all-in-one, affordable and pain-free solution to integrate text to speech with a UK English accent, one that only weighs 14kB and solves the myriad problems speech synthesis entails, which include (but unfortunately are not limited to) per-character costs, having to initialise the speech engine after page load, timing delays between API calls, speech rate problems, it’s a mess we chose to tidy up so you don’t have to! UK English text to speech with ResponsiveVoice Compatibility It's that easy to get your audio file to translated.UK English text to speech needs good technology to work well, but that technology is difficult to come by, and it’s always a pain to implement. Click on "Export" and choose your preferred file format. Our audio translator will generate the translation of your transcript within a minute. Select "Translation" and choose your language. If you choose our human service, your transcript will be ready within 24 hours. Our automatic transcription software will convert your file to text in just a few minutes (depending on the length of your file). With our human service, your file will be transcribed and proofread by an expert and native speaker and delivered with 99% accuracy. Our automatic transcription software is lightning fast and 85% accurate. Choose "Machine generated" or "Human made". ![]() To translate your audio, we first need to transcribe it. We support transcription in 120 languages, dialects, and accents. The first 10 minutes are free and there's no file limit. ![]() Download the app to explore the world and communicate with people across many languages. ![]() With our uploader, you can import your file from anywhere, whether it's on your laptop, Google Drive, Youtube, or Dropbox. Start using Google Translate in your browser.Or scan the QR code below to download the app to use it on your mobile device. ![]() ![]() ![]() The following article focuses on accounting and bookkeeping. Keep in mind that bookkeepers don’t interrupt and analyze data. On the contrary, accounting is the process of summarizing, analyzing, classifying, and reporting the company’s financial data. Bookkeeping can be defined best as keeping all financial transactions of the business on track. In the above table, you can see the differences between accounting and bookkeeping where book keeping ends.The two essential aspects of a business organization are bookkeeping and accounting as its crucial functions. Hope, now you understand that what is the difference between bookkeeping and accounting. The financial position of the company cannot be determined through bookkeeping. Through accounting, the user can easily determine the financial position of the entity. Single Entry Bookkeeping Double Entry Bookkeeping Profit and loss statement, Balance Sheet and Cash Flow Statement The user cannot make decisions on the basis of bookkeepingįinancial statements are prepared in accounting.Īs bookkeeping is the part of accounting, it does not deal with the financial statement preparation. The user can make decisions on the basis of accounting Under bookkeeping company’s financial transaction are recorded in a systemized manner. Now, we come to the difference between bookkeeping and accounting and accountancy in tabular form.Īccounting is recording, classifying, summarizing and classifying of the financial transaction of business. After this, the financial statements are intensively analyzed and ends with providing such reports to the prospective users. ![]() Thereafter, such classified transactions are summarized in a manner that can be presented to the existing user of the company. In accounting the financial transaction of an association are recognized and periodically recorded, after that they are classified and grouped. Accounting begins with the financial transaction recording and ends on financial statement reporting to the users at the end of the year to give a true and fair view of the business. There are two ways to update the books of accounts and they are:Īccounting is also known as the business language that provides information regarding the financial status of the business. ![]() And posts them in the concerned ledger, after that transferred in the trial balance. The bookkeeper records the transactions in the books of accounts like sales, sales return, purchase, purchase return, journal and so forth. Some of the common examples of day to day transactions are goods sold, goods purchased, goods returned and so forth. The bookkeeper is the one who looks after that each financial transaction of the business is recorded regularly and systematically. Moreover, bookkeeping helps in finding the true and fair picture of profit and loss statement at the end of the accounting period. ![]() It helps in recording each and every transaction of the business so as to form the base for accounting. The procedure of complete and efficient record keeping of the transaction of an association by the bookkeeper is called bookkeeping. Take a look at the article, which shows the difference between bookkeeping and accounting: Meaning of Bookkeeping Moreover, accounting needs specialized knowledge of the subject, analytical skill, conceptual understanding and so on. On the contrary, accounting is related to the classifying, analyzing and summarizing the recorded monetary transaction. Through this, we can conclude that the job of a bookkeeper is clerical in nature. While bookkeeping, on the other hand, is related to updating the financial transaction in the books of accounts. Bookkeeping helps in forming the base for accounting as it is just a part of accounting. Moreover, accounting is a lot more analytical and extensive than bookkeeping. Many utilize the bookkeeping and accounting terms reciprocally, yet the matter of the fact is that bookkeeping is a part of accounting. Let's Know Book Keeping and Accountancy Difference ![]() |
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